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The winners of the 47th Nikon Small World Photomicrography Competition have been announced, with the top prize awarded to the Baylor College of Medicine's Jason Kirk for his close-up image of a southern live oak leaf.
Stacking together 200 individual images of the leaf, Kirk used a custom-made microscope system and edited the colour temperature and hue in post-production. Those white tentacle looking things are trichomes (which protect the plant against extreme weather), the purple parts are the stomata (which regulate gases), and the bits in cyan are vessels (which guide water through the plant).
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"The lighting side of it was complicated,” said Kirk in a press statement. "Microscope objectives are small and have a very shallow depth of focus. I couldn’t just stick a giant light next to the microscope and have the lighting be directional. It would be like trying to light the head of a pin with a light source that's the size of your head. Nearly impossible.”
SEE ALSO: Stunning microscopic videos illuminate some of Earth’s deeply hidden mysteriesEsmeralda Paric won second place for her image of a microfluidic device filled with 300,000 networking neurons, and Frank Reiser won third for his close-up image of a hog louse.
These images below are the top 20, in order, from the photography component of the competition, which also features an exceptional video prize — you need to see the winners of this year's comp. Plus, if you'd like more, here are last year's winners.
Winner: Jason Kirk, Trichome (white appendages) and stomata (purple pores) on a southern live oak leaf. Credit: Jason Kirk 
Second place: Esmeralda Paric and Holly Stefen, A microfluidic device containing 300,000 networking neurons in two isolated populations. Both sides were treated with a unique virus and bridged by axons. Credit: Esmeralda Paric and Holly Stefen 
Third place: Frank Reiser, Rear leg, claw, and respiratory trachea of a louse. Credit: Frank Reiser 
Paula Diaz, Sensory neuron from an embryonic rat. Credit: Paula Diaz 
Oliver Dum, Proboscis of a housefly. Credit: Oliver Dum 
Dr. Andrea Tedeschi, 3D vasculature of an adult mouse brain (somatosensory cortex). Credit: DR. ANDREA TEDESCHI 
Dr. Tong Zhang and Dr. Paul Stoodley, Head of a tick. Credit: Dr. Tong Zhang and Dr. Paul Stoodley 
Dr. Amy Engevik, Cross section of mouse intestine. Credit: Dr. Amy Engevik 
Jan van IJken, Water flea carrying embryos and peritrichs. Credit: Jan van IJken 
Sébastien Malo, Vein and scales on a butterfly wing. Credit: Sébastien Malo 
Jason Kirk and Carlos P. Flores Suarez, Vasculature of a mouse retina. Credit: Jason Kirk and Carlos P. Flores Suarez 
Jakub Sumbal, Breast organoid showing contractile myoepithelial cells (blue) crawling on secretory breast cells (red). Credit: Jakub Sumbal 
Dr. Felice Placenti, Cotton fabric with pollen grains. Credit: Dr. Felice Placenti 
Dr. Joern N. Hopke, Snowflake. Credit: Dr. Joern N. Hopke 
Bernard Allard, Diatom (Arachnoidiscus). Credit: Bernard Allard 
Ruohan Zhong, An in vivo snapshot of the neurons surrounding the mouth and tentacles of a juvenile starlet sea anemone. Credit: Ruohan Zhong 
Martin Kaae Kristiansen, Filamentous strands of Nostoc cyanobacteria captured inside a gelatinous matrix. Credit: Martin Kaae Kristiansen 
Saulius Gugis, Table salt crystal. Credit: Saulius Gugis 
Billie Hughes, Calcite crystal inclusion suspended in a spinel gemstone. Credit: Billie Hughes 
Alison Pollack, Slime mold (Arcyria pomiformis). Credit: Alison Pollack Want more close-ups under the microscope? Check out the video winners.